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Spalters Inc. #5: Second attempt preliminary results: win

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Blog entry by Sodabowski posted 1169 days ago 4100 reads 0 times favorited 13 comments Add to Favorites Watch
« Part 4: Second culture attempts Part 5 of Spalters Inc. series Part 6: determining the temperature of destruction of xylindein »

Hey guys,

Out of the six compartments that I inoculated with Chlorociboria Aeruginosa in my petri dishes, one has absolutely settled and started growing and producing xylindein. Still I have to clean them every few days as the molds that invited themselves to the party are tough to fight, so I opted for the “gardening” method and just mow them out when they become too invasive. Using a lab culture medium would have prevented that from happening, but as I already stated in the previous post, the price is insane.

Say hello to my little friend!

I haven’t finished the culture box yet, lack some tools that are at the parents’.

TTYL

-- Holy scrap Barkman!



13 comments so far

View Schwieb's profile

Schwieb

1493 posts in 2086 days


#1 posted 1169 days ago

Congratulations on your success thus far. I admire your tenacity.

-- Dr. Ken, Florida - Durch harte arbeit werden Träume wahr.

View Bertha's profile

Bertha

12951 posts in 1318 days


#2 posted 1169 days ago

Sodabowski, you’ve done it. You’ve finally completed the entire circle for me. I’m an industrial microbiologist by training, and MD to pay the bills. I’ve worked as a brewer (yeast bank director) and cell culturer. What you’re doing right now completes some obscure circle for me. :) Thank you for this.

-- My dad and I built a 65 chev pick up.I killed trannys in that thing for some reason-Hog

View Sodabowski's profile

Sodabowski

2002 posts in 1458 days


#3 posted 1169 days ago

@ Ken: I promised you some of this green-sytained wood and I will keep my promise whatever it takes ;)

@Al: I feel very honored by your comment mate :) I want to and will harness this lil’ fella!

-- Holy scrap Barkman!

View llwynog's profile

llwynog

283 posts in 1203 days


#4 posted 1169 days ago

Thomas,
Congratulations on the birth of your numerous children !

-- Fabrice - "On est bien bête mais on sent bien quand on se fait mal" - my grandfather

View chrisstef's profile

chrisstef

10621 posts in 1631 days


#5 posted 1169 days ago

We’ve done it Igor, we’ve done it !!! Can we call it Spaltenstein?

-- "there aren’t many hand tools as awe-inspiring as the #8 jointer. I mean, it just reeks of cast iron heft and hubris" - Smitty

View Sodabowski's profile

Sodabowski

2002 posts in 1458 days


#6 posted 1169 days ago

Bwahaha ^^

-- Holy scrap Barkman!

View Manitario's profile

Manitario

2298 posts in 1508 days


#7 posted 1169 days ago

As an MD with a microbiology background, and having spent a few years working with cell cultures, this series fulfills my inner nerd. As Al put it, it completes some sort of strange circle. Seriously, this is one of the most interesting things I’ve read on LJ’s. Not sure how much malt agar extract you’d need but you may want to check out sigmaaldrich.com
A lot of their products are shipped from Europe anyways, you may be able to pick up the agar medium for cheaper than Ebay.

-- Sometimes the creative process requires foul language. -- Charles Neil

View Sodabowski's profile

Sodabowski

2002 posts in 1458 days


#8 posted 1169 days ago

Thanks for the nice words Rob, and also for the link! I’m pretty sure I will spend some cash there, hehehe.
I just saw that the guys at Sigma also have everything needed for chromatography, which is perfect for me as I also plan to do some hardcore dissociation of everything I can extract off of greened wood (I actually made a first attempt but I need my chems which are at the parents’ ).

As I stated earlier on the series, on my first culture experiment I got a yellow fungus running like crazy alongside Chlorociboria, and here a chromatography could bring very interesting results as to exactly what color components are present in the colored wood. My specimens are emerald green, but I know of franckly blue ones, so why not give it a try and see what comes out :)

-- Holy scrap Barkman!

View Manitario's profile

Manitario

2298 posts in 1508 days


#9 posted 1169 days ago

Chromatography! You certainly have some interesting hobbies. I haven’t thought about chromatography since undergrad chemistry. If I ever make it to Paris, I’ll have to pay you a visit and check out some of these projects.

-- Sometimes the creative process requires foul language. -- Charles Neil

View Sodabowski's profile

Sodabowski

2002 posts in 1458 days


#10 posted 1169 days ago

Trade maple syrup for spalting fungi? ;)

BTW I actually used maple syrup in this growing attempt: so far, so good.

I’ll have to build a decent chromatography system then! my first attempt was made of two nutella lids with a aper tissue in between, all in a japanese takeaway food container lid, hahaha, not quite worthy of being shown off to the world ^^

-- Holy scrap Barkman!

View mafe's profile

mafe

9483 posts in 1714 days


#11 posted 1169 days ago

Thomas you did it, gave birth to a little blue.
Congrat I can’t wait to see where this will go.
Best thoughts,
Mads

-- Mad F, the fanatical rhykenologist and vintage architect. Democraticwoodworking.

View Leonard5's profile

Leonard5

317 posts in 1318 days


#12 posted 1154 days ago

Cool

-- Leonard H.

View EPJartisan's profile

EPJartisan

1052 posts in 1750 days


#13 posted 1151 days ago

Alright!! This also excites me.. how odd that is. I haven’t checked in a while but after your posts I fortified my spalting efforts with beer.. if I have no improvement … I will add honey to the plastic bin.

-- " 'Truth' is like a beautiful flower, unique to each plant and to the season it blossoms ... 'Fact' is the root and leaf, allowing the plant grow and bloom again."

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