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Spalters Inc. #5: Second attempt preliminary results: win

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Blog entry by Sodabowski posted 713 days ago 2395 reads 0 times favorited 13 comments Add to Favorites Watch
« Part 4: Second culture attempts Part 5 of Spalters Inc. series Part 6: determining the temperature of destruction of xylindein »

Hey guys,

Out of the six compartments that I inoculated with Chlorociboria Aeruginosa in my petri dishes, one has absolutely settled and started growing and producing xylindein. Still I have to clean them every few days as the molds that invited themselves to the party are tough to fight, so I opted for the “gardening” method and just mow them out when they become too invasive. Using a lab culture medium would have prevented that from happening, but as I already stated in the previous post, the price is insane.

Say hello to my little friend!

I haven’t finished the culture box yet, lack some tools that are at the parents’.

TTYL

-- Thomas - There is no such thing as a problem, there only are solutions.





13 comments so far

View Schwieb's profile

Schwieb

1108 posts in 1630 days


#1 posted 713 days ago

Congratulations on your success thus far. I admire your tenacity.

-- Dr. Ken, Florida - Durch harte arbeit werden Träume wahr.

View Bertha's profile

Bertha

13111 posts in 862 days


#2 posted 713 days ago

Sodabowski, you’ve done it. You’ve finally completed the entire circle for me. I’m an industrial microbiologist by training, and MD to pay the bills. I’ve worked as a brewer (yeast bank director) and cell culturer. What you’re doing right now completes some obscure circle for me. :) Thank you for this.

-- My dad and I built a 65 chev pick up.I killed trannys in that thing for some reason-Hog

View Sodabowski's profile

Sodabowski

1807 posts in 1002 days


#3 posted 713 days ago

@ Ken: I promised you some of this green-sytained wood and I will keep my promise whatever it takes ;)

@Al: I feel very honored by your comment mate :) I want to and will harness this lil’ fella!

-- Thomas - There is no such thing as a problem, there only are solutions.

View llwynog's profile

llwynog

231 posts in 747 days


#4 posted 713 days ago

Thomas,
Congratulations on the birth of your numerous children !

-- Fabrice - "On est bien bête mais on sent bien quand on se fait mal" - my grandfather

View chrisstef's profile

chrisstef

5253 posts in 1175 days


#5 posted 713 days ago

We’ve done it Igor, we’ve done it !!! Can we call it Spaltenstein?

-- "there aren’t many hand tools as awe-inspiring as the #8 jointer. I mean, it just reeks of cast iron heft and hubris" - Smitty

View Sodabowski's profile

Sodabowski

1807 posts in 1002 days


#6 posted 713 days ago

Bwahaha ^^

-- Thomas - There is no such thing as a problem, there only are solutions.

View Manitario's profile

Manitario

1862 posts in 1052 days


#7 posted 713 days ago

As an MD with a microbiology background, and having spent a few years working with cell cultures, this series fulfills my inner nerd. As Al put it, it completes some sort of strange circle. Seriously, this is one of the most interesting things I’ve read on LJ’s. Not sure how much malt agar extract you’d need but you may want to check out sigmaaldrich.com
A lot of their products are shipped from Europe anyways, you may be able to pick up the agar medium for cheaper than Ebay.

-- Rob, Sault Ste. Marie, Ontario

View Sodabowski's profile

Sodabowski

1807 posts in 1002 days


#8 posted 713 days ago

Thanks for the nice words Rob, and also for the link! I’m pretty sure I will spend some cash there, hehehe.
I just saw that the guys at Sigma also have everything needed for chromatography, which is perfect for me as I also plan to do some hardcore dissociation of everything I can extract off of greened wood (I actually made a first attempt but I need my chems which are at the parents’ ).

As I stated earlier on the series, on my first culture experiment I got a yellow fungus running like crazy alongside Chlorociboria, and here a chromatography could bring very interesting results as to exactly what color components are present in the colored wood. My specimens are emerald green, but I know of franckly blue ones, so why not give it a try and see what comes out :)

-- Thomas - There is no such thing as a problem, there only are solutions.

View Manitario's profile

Manitario

1862 posts in 1052 days


#9 posted 713 days ago

Chromatography! You certainly have some interesting hobbies. I haven’t thought about chromatography since undergrad chemistry. If I ever make it to Paris, I’ll have to pay you a visit and check out some of these projects.

-- Rob, Sault Ste. Marie, Ontario

View Sodabowski's profile

Sodabowski

1807 posts in 1002 days


#10 posted 713 days ago

Trade maple syrup for spalting fungi? ;)

BTW I actually used maple syrup in this growing attempt: so far, so good.

I’ll have to build a decent chromatography system then! my first attempt was made of two nutella lids with a aper tissue in between, all in a japanese takeaway food container lid, hahaha, not quite worthy of being shown off to the world ^^

-- Thomas - There is no such thing as a problem, there only are solutions.

View mafe's profile

mafe

8055 posts in 1258 days


#11 posted 713 days ago

Thomas you did it, gave birth to a little blue.
Congrat I can’t wait to see where this will go.
Best thoughts,
Mads

-- Mad F, the fanatical rhykenologist and vintage architect. Democraticwoodworking.

View Leonard5's profile

Leonard5

192 posts in 862 days


#12 posted 698 days ago

Cool

-- Leonard H.

View EPJartisan's profile

EPJartisan

804 posts in 1294 days


#13 posted 695 days ago

Alright!! This also excites me.. how odd that is. I haven’t checked in a while but after your posts I fortified my spalting efforts with beer.. if I have no improvement … I will add honey to the plastic bin.

-- ~ Eric P Jorgenson: Jorgenson Design

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